Yes. You can put lead files into different campaigns to organize them. Some clients organize lead files by source, by target product, or by location. You don't need to have multiple campaigns, however, and if you are only selling one product, it may be easier to upload all leads into a single campaign, and create multiple playlists containing newer or older files to be called by different agents if you so choose.
Having one campaign instead of multiple may be more suitable for your specific business because if you upload a lead to one campaign, and then you happen to upload that same lead to another campaign, the system will not reject duplicated leads across different campaigns by default, which could cause confusion moving forward.
Eg. If you disposition the lead as an appointment for a later date, the dialer could still call that lead, again, before your appointment, because it would be calling it from a different campaign.
Leads are marked by ID tags, and when a lead is placed into the system twice in this manner, it will create a secondary lead ID tag. You can, however, ask for a CRM tweak to be turned on that will force the system to search all campaigns for any lead being uploaded. This would help prevent the duplication of a lead.